Packages (Simulation)
Image (II)
-
Figure. SDS-PAGE
Quality Guarantee
Instruction Manual
Certificate
Active Complement Component 3a (C3a)
- Product No.APA387Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point8.2
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 188
US$ 470
US$ 940
US$ 2820
US$ 7050
For more details, please contact local distributors!
ACTIVITY TEST
Complement Component 3a (C3a) is a component of the complement system, this protein is also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. It's been identified that C3A can interact with Complement Factor I (CFI), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C3a and recombinant human CFI. Briefly, C3a was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C3a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C3a and recombinant human CFI was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| 22 | Immobilization of soluble complement receptor 1 on islets. ScienceDirect: S0142961211002596 |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845 |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587 |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977 |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247 |
| Am J Reprod Immunol | Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study PubMed: 26388133 |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358 |
| Plos One | Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice Pubmed:26872035 |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
| Colloids Surf B Biointerfaces. | Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. pubmed:28131092 |
| Pediatric Pulmonology | Accuracy of complement activation product levels to detect infected pleural effusion in rats pubmed:28474458 |
| Journal of Immunology | The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression Pubmed:29367209 |
| Artificial Cells Nanomedicine and Biotechnology Journal | Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly … Pubmed:29873524 |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation Doi: 10.1016/j.jcyt.2018.07.007 |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976 |
| Clinical Immunology | Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss |
| Materials Science and Engineering: C | Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys |
| Nanomedicine | Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion 33549818 |
| EXPERIMENTAL EYE RESEARCH | Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression 33493474 |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |