≡

Active Elastin Microfibril Interface Located Protein 1 (EMILIN1)

EMI; gp115; Elastin Microfibril Interfacer 1

ACTIVITY TEST

Elastin Microfibril Interface Located Protein 1 (EMILIN1) is a glycoprotein that plays an important role in the extracellular matrix (ECM). It is widely present in the extracellular matrix of arteries, lymphatic vessels and other tissues. EMILIN1 plays an important role in several biological processes, such as participating in the biosynthesis of elastic fibers (elastogenesis), maintaining vascular cell morphology and regulating cell signaling. Besides, Integrin Beta 1 (ITGb1) has been identified as an interactor of EMILIN1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EMILIN1 and recombinant human ITGb1. Briefly, EMILIN1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EMILIN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EMILIN1 and recombinant human ITGb1 was shown in Figure 1, the EC50 for this effect is 6.64 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA415Hu01 Recombinant Elastin Microfibril Interface Located Protein 1 (EMILIN1) Positive Control; Immunogen; SDS-PAGE; WB.
APA415Hu01 Active Elastin Microfibril Interface Located Protein 1 (EMILIN1) Cell culture; Activity Assays.
PAA415Hu01 Polyclonal Antibody to Elastin Microfibril Interface Located Protein 1 (EMILIN1) WB; IHC; ICC; IP.
MAA415Hu22 Monoclonal Antibody to Elastin Microfibril Interface Located Protein 1 (EMILIN1) WB; IHC; ICC; IP.
SEA415Hu ELISA Kit for Elastin Microfibril Interface Located Protein 1 (EMILIN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA415Hu Multiplex Assay Kit for Elastin Microfibril Interface Located Protein 1 (EMILIN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.