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Active Tissue Plasminogen Activator (tPA)

PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase

ACTIVITY TEST

Figure. The binding activity of tPA with PAI1.
Plasminogen activators are serine proteases that catalyze the activation of plasmin via proteolytic cleavage of its zymogen form plasminogen. Plasmin is an important factor in fibrinolysis, the breakdown of fibrin polymers formed during blood clotting. There are two main plasminogen activators: urokinase (uPA) and tissue plasminogen activator (tPA).Tissue plasminogen activators (tPA) are used to treat medical conditions related to blood clotting including embolic or thrombotic stroke, myocardial infarction, and pulmonary embolism. Besides, Plasminogen Activator Inhibitor 1 (PAI1) has been identified as an interactor of tPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse tPA and recombinant mouse PAI1. Briefly, tPA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PAI1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-tPA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of tPA and PAI1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Annals of Clinical & Laboratory Science Alteration of Hemostatic Parameters in Patients with Different Levels of Subclinical Hypothyroidism and the Effect of L-thyroxine Treatment pubmed:28249913
Cellular Physiology and Biochemistry Defective Platelet Activation and Bleeding Complications upon Cholestasis in Mice pubmed:28441661
Journal of allergy and clinical immunology Cytokine and estrogen stimulation of endothelial cells augments activation of the prekallikrein-high molecular weight kininogen complex: Implications for hereditary angioedema. pubmed:27826093
European Journal of Neuroscience Urokinase receptor and tissue plasminogen activator as immediate‐early genes in pentylenetetrazole‐induced seizures in the mouse brain Pubmed: 31587391
Phytomedicine Sodium aescinate significantly suppress postoperative peritoneal adhesion by inhibiting the RhoA/ROCK signaling pathway Pubmed: 32120245
Sci Rep Fibroblast growth factor-21 as a novel metabolic factor for regulating thrombotic homeostasis Pubmed:35013379
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA525Mu01 Active Tissue Plasminogen Activator (tPA) Cell culture; Activity Assays.
RPA525Mu02 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Mu01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA525Mu01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
PAA525Mu02 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
LAA525Mu71 Biotin-Linked Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC.
SEA525Mu ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA525Mu CLIA Kit for Tissue Plasminogen Activator (tPA) Chemiluminescent immunoassay for Antigen Detection.
LMA525Mu Multiplex Assay Kit for Tissue Plasminogen Activator (tPA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.