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Active Myelin Basic Protein (MBP)
Myelin A1 protein; Myelin membrane encephalitogenic protein
- Product No.APA539Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point9.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 202
US$ 504
US$ 1008
US$ 3024
US$ 7560
For more details, please contact local distributors!
ACTIVITY TEST
Myelin basic protein (MBP), a 18.5-kDa encephalitogenic peptide, is the second most abundant in central nervous system and responsible for adhesion of the cytosolic surfaces of multilayered compact myelin, MBP is biased expressed in cerebellum adult and cortex adult. It can interact with a number of polyanionic proteins including actin, tubulin, Ca(2 )-calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. Calmodulin Like Protein 3 (CALML3) is a kind of Ca(2 )-calmodulin, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MBP and recombinant human CALML3. Briefly, biotin-linked MBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALML3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinan human MBP and recombinan human CALML3 was shown in Figure 1, the EC50 for this effect is 7.4 ug/mL.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source |
| Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain Pubmed: 23183024 |
| Pak J Med Sci | Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke cases PubMed: 26648996 |
| Experimental and Toxicologic Pathology | Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus) Pubmed:27165445 |
| Acta Angiologica | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008 |
| Stem Cells. | Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. pubmed:27573615 |
| Environmental Science and Pollution Research | Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures. pubmed:27696165 |
| Acta Angiol | Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomy AA.2016.0008 |
| PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis pubmed:28489854 |
| Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats Pubmed:30033014 |
| International Journal of Livestock Research | Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies 10.5455:ijlr.20170811080455 |
| Microchimica Acta | Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a review Pubmed: 31760523 |
| The role of light desynchronosis in the development of stress-induced aging | |
| Adv Med Sci | Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring 34091432 |
| biomolecules | Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡ |
| Talanta | Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in … Pubmed:35202838 |