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Active Matrix Metalloproteinase 9 (MMP9)

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

ACTIVITY TEST

Mechanism:MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family . It is a gelatinase A, 92 kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10 pg of MMP-2 can already be detected .Briefly，various concentrations of recombinant mouse MMP9 (100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.1ng, 1.5ng and 0.7ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 15% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use trypsin as positive control.
Result: Gelatin hydrolysis by recombinant mouse MMP9(10-70kd) was shown in figure 1.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
EPA553Mu51 Eukaryotic Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
APA553Mu01 Active Matrix Metalloproteinase 9 (MMP9) Cell culture; Activity Assays.
APA553Mu61 Active Matrix Metalloproteinase 9 (MMP9) Cell culture; Activity Assays.
APA553Mu62 Active Matrix Metalloproteinase 9 (MMP9) Cell culture; Activity Assays.
EPA553Mu62 Eukaryotic Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
EPA553Mu61 Eukaryotic Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
RPA553Mu01 Recombinant Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
RPA553Mu02 Recombinant Matrix Metalloproteinase 9 (MMP9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA553Mu02 Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
PAA553Mu01 Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; ICC/IF
PAA553Mu07 Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC; IP.
LAA553Mu71 Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) WB; IHC; ICC.
SEA553Mu ELISA Kit for Matrix Metalloproteinase 9 (MMP9) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA553Mu ELISA Kit for Anti-Matrix Metalloproteinase 9 Antibody (Anti-MMP9) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA553Mu CLIA Kit for Matrix Metalloproteinase 9 (MMP9) Chemiluminescent immunoassay for Antigen Detection.
LMA553Mu Multiplex Assay Kit for Matrix Metalloproteinase 9 (MMP9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.