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Active S100 Calcium Binding Protein B (S100B)

S100-B; NEF; S100Beta; S-100 protein subunit beta

ACTIVITY TEST

Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Epilepsia Protective effects of naloxone in two-hit seizure model PubMed: 19674055
Journal of Neurotrauma The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats Liebert: 20091210
Food and Chemical Toxicology Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal PubMed: 20637253
20 Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder Wiley: source
Neuroscience Letters A preliminary study of the effects of ulinastatin on early postoperative cognition function in patients undergoing abdominal surgery Pubmed: 23422155
Genes Cells Induction of receptor for advanced glycation end products by insufficient leptin action triggers pancreatic β-cell failure in type 2 diabetes. Pubmed: 23410183
J Craniofac Surg. The Experiment Research of Neuroprotection of Hypoperfusion Postconditioning on Cerebral Ischemia Pubmed:25006960
Int J Mol Sci The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions PubMed: 26184156
Mol Neurobiol The Severity of Cecal Ligature and Puncture-Induced Sepsis Correlates with the Degree of Encephalopathy, but the Sepsis Does Not Lead to Acute Activation of Spleen Lymphocytes in Mice PubMed: 26081155
Pediatria i Medycyna Rodzinna Stężenie białka S100B w surowicy krwi u młodzieży z omdleniami yadda:60e5630d0ac1
中国临床药理学与治疗学 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 article:9794
Eur J Pharmacol.  Development of an experimental rat model of hyperammonemic encephalopathy and evaluation of the effects of rifaximin Pubmed:26980242
Journal For Neurovirology Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. pubmed:27882497
Neurourol Urodyn Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid pubmed:27490041
cellular physiology and biochemistry Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … pubmed:29040978
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286
Brain Research Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway Pubmed:29510144
Neurochemical Research Protective Effects of Cornel Iridoid Glycoside in Rats After Traumatic Brain Injury Pubmed:29492766
Biochemical and Biophysical Research Communications C6 glioma-conditioned medium induces malignant transformation of mesenchymal stem cells: Possible role of S100B/RAGE pathway Pubmed:29050939
Journal of the Neurological Sciences S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … Pubmed:29801873
Biochemistry and Cell Biology Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy Pubmed:29365285
S100B as a potential biomarker for concussion in male and female collegiate rugby athletes :
Indian Journal of Psychiatry Serum S100B levels in patients with depression
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
Brain Sciences Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells Pubmed: 31013844
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
ACTA NEUROBIOLOGIAE EXPERIMENTALIS Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … Pubmed: 32214270
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366
Neurocritical Care Biomarkers of Neurological Outcome After Aneurysmal Subarachnoid Hemorrhage as Early Predictors at Discharge from an Intensive Care Unit Pubmed: 32978732
European Review for Medical and Pharmacological Sciences Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … Pubmed: 32432773
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …
Scientific Reports Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD) 33469081
Crit Care Serum biomarkers and cerebral autoregulation as early warnings of delayed cerebral ischemia risk in patients after aneurysmal subarachnoid haemorrhage 27885969
Transl Psychiatry Increased GFAP concentrations in the cerebrospinal fluid of patients with unipolar depression 34021122
Biochimie Neuroprotective effect of 6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline mediated via regulation of antioxidant system and inhibition of inflammation and apoptosis in?¡­ 33964368
Int J Neuropsychopharmacol High S100B levels predict antidepressant response in patients with major depression even when considering inflammatory and metabolic markers Pubmed:35176771
Metab Brain Dis 1-benzoyl-6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline exerts a neuroprotective effect and normalises redox homeostasis in a rat model of cerebral ischemia … Pubmed:35201554
PLoS One Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats Pubmed:35421180
International Journal of Molecular Sciences Sepsis Encephalopathy Is Partly Mediated by miR370-3p-Induced Mitochondrial Injury but Attenuated by BAM15 in Cecal Ligation and Puncture Sepsis Male Mice Pubmed:35628259
Authorea THE EVALUATION OF MEDULLA SPINALIS PERFUSION AT MODERATE HYPOTHERMIA WITH PATIENTS WHO UNDERGO ANTEGRADE SELECTIVE CEREBRAL …
EPIDEMIOLOGIA, DETERMINANTELE ȘI PREDICȚIA ACCIDENTULUI VASCULAR CEREBRAL ISCHEMIC LA COPII
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