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Active Adiponectin (ADPN)

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

ACTIVITY TEST

Human Adiponectin (ADPN) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is a plasma protein secreted by adipose tissue showing pleiotropic effects with anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin and AdipoR1 regulate PPARgC1a and mitochondria by Ca(2 ) and AMPK/SIRT1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken ADPN and recombinant rat PPARgC1a. Briefly, ADPN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken ADPN and recombinant rat PPARgC1a was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
BioMed Research International Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats PubMed: PMC3591142
PLoS One CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies PubMed: PMC3297623
Molecular and Cellular Endocrinology Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194
British Journal of Nutrition n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577
International Urology Nephrology Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332
Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781
Mol Biol Rep. Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. Pubmed: 24178343
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658
Iran J Basic Med Sci Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234
Biometals.? Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243
Poult Sci.? Effect of perches on liver health of hens Pubmed:24812236
Gen Comp Endocrinol. The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats Pubmed:25019654
Journal of Trace Elements in Medicine and Biology Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956
J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Int J Clin Exp Pathol Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802
Cell Physiol Biochem Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485
Life Sciences Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592
MEDICAL RESEARCH JOURNAL Relation between afamin level and some inflammatory markers in obese children Mrjnrc: Abstract
Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925
Technologia Alimentaria Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10
Obes Surg. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
Adv Biomed Res. Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393
Biological Trace Element Research Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats article:s12011-016-0777-1
Journal of Functional Foods Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans science:S1756464616300585
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
Journal of Molecular and Cellular Cardiology Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193
Journal of Diabetes Research Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795
Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817
Biomedical and Pharmacology Journal Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … vol10no1
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats pubmed:29147484
Evidence-Based Complementary and Alternative Medicine BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway journals:1758383
Oxidative Medicine and Cellular Longevity Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis journals:3405089
Iranian Journal of Pharmaceutical Research Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance pubmed:29201096
Biomedical & Pharmacology Journal Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study DOI:10.13005/bpj/1123
Oncotarget FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696
Frontiers in Endocrinology Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407
Food & Function Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese mice Pubmed:29595858
Evidence-based Complementary and Alternative Medicine Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … Pubmed:29619063
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010
Veterinary immunology and immunopathology Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia Pubmed:30078593
Hematology Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Pubmed:29318945
International Journal of Gerontology Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats 10.1016:j.ijge.2018.01.002
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120
J Bas Res Med Sci Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats article-1-352-en.pdf
Iraq Medical Journal Adiponectin may be used as a marker in prediction of diabetic retinopathy patients JOCMS:Source
Food Science and Technology Research Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells Doi: 10.3136/fstr.24.903
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783
Nutrients Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet Pubmed: 30400147
Progress in Nutrition Effect of fermented food consumption on biochemical parameters and adipokines levels Doi: 0.23751/pn.v20i4.7374
Biology of Reproduction Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway Pubmed: 30496353
Nutrition in the Prevention and Treatment of Abdominal Obesity Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig
Evidence-Based Complementary and Alternative Medicine BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice
Life Sciences Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats Pubmed: 30826497
Arteriosclerosis, Thrombosis, and Vascular Biology Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo Pubmed: 30816801
Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP … Pubmed: 31003008
Gene Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension Pubmed: 30940525
中国药理学通报 冷暴露对高脂饮食小鼠脂肪棕色化的影响
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
Proceedings of the Royal Society B: Biological Sciences Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054
Cytokine Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Pubmed: 31514105
Food & Function Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice Pubmed: 31670366
journal of ethnopharmacology Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Pubmed: 31756448
Annals of Nutrition and Metabolism Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561
Journal of Hazardous Materials Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice Pubmed: 31796355
Experimental and Clinical Endocrinology & Diabetes HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome Pubmed: 32189318
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation Pubmed: 32227855
Nature Communications AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis Pubmed: 33199780
Metabolites Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia Pubmed: 33066473
Ann Nutr Metab Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789
aging Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats Pubmed: 32970613
Diabetes Metab Syndr Obes Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population Pubmed: 32904655
DIABETOLOGIA Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … Pubmed: 32880686
J Endocrinol Invest Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity Pubmed: 32633953
J Endocrinol Invest Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats Pubmed: 32588381
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480
Diabetes Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling Pubmed: 32409493
Journal of Cellular and Molecular Medicine Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats Pubmed: 32395890
The Egyptian Journal of Chest Diseases and Tuberculosis Study of serum adiponectin and resistin in patients with obstructive sleep apnea
Biosciences Biotechnology Research Asia Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus
Computational Biology and Chemistry In-silico therapeutic investigations of arjunic acid and arjungenin as an FXR agonist and validation in 3T3-L1 adipocytes
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274
Cell Metab TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease 34107313
Biomedical and Environmental Sciences Decreased Plasma MANF Levels are Associated with Type 2 Diabetes 33766221
International Journal of Endocrinology Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­ 34035808
International Journal of Pharmacy and Biological Sciences Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity
Journal of Functional Foods Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women
PPAR Research Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … 34306045
Br J Nutr The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809
Phytomedicine Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice 34561124
Biofactors Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670
Kaohsiung J Med Sci Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients 34672426
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313
Animals Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study 34438765
Cell Death & Disease Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance Pubmed:35347118
Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India Pubmed:35717896
BioMed Research International Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment Pubmed:35087911
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Scientific Reports SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA605Ga01 Recombinant Adiponectin (ADPN) Positive Control; Immunogen; SDS-PAGE; WB.
APA605Ga01 Active Adiponectin (ADPN) Cell culture; Activity Assays.
PAA605Ga01 Polyclonal Antibody to Adiponectin (ADPN) WB; IHC; ICC; IP.
MAA605Ga21 Monoclonal Antibody to Adiponectin (ADPN) WB; IHC; ICC; IP.
SEA605Ga ELISA Kit for Adiponectin (ADPN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA605Ga CLIA Kit for Adiponectin (ADPN) Chemiluminescent immunoassay for Antigen Detection.
LMA605Ga Multiplex Assay Kit for Adiponectin (ADPN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.