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Active Neuropilin 1 (NRP1)

CD304; BDCA4; VEGF165R; Vascular endothelial cell growth factor 165 receptor

ACTIVITY TEST

Neuropilin 1 (NRP1), as kown as CD304 or VEGF165R, is a 130 - 140 kDa type I transmembrane (TM) glycoprotein. NRP1 is expressed by neurons, blood vessels, immune cells and many other cell types in the mammalian body and binds a range of structurally and functionally diverse extracellular ligands to modulate organ development and function. VEGF165, an angiogenic cytokine, has higher affinity for NRP1. A functional binding ELISA assay was conducted to detect the interaction of recombinant human NRP1 and recombinant human VEGF165. Briefly, NRP1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VEGF165-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NRP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human NRP1 and recombinant human VEGF165 was shown in Figure 1, the EC50 for this effect is 2.98 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
APMIS Peritumoral brain edema in angiomatous supratentorial meningiomas: an investigation of the vascular endothelial growth factor A pathway PubMed: 23398358
Disease Markers Circulating Soluble Neuropilin-1 in Patients with Early Cervical Cancer and Cervical Intraepithelial Neoplasia Can Be Used as a Valuable Diagnostic Biomarker Pubmed:25873749
JOMO KENYATTA UNIVERSITY OF AGRICULTURE AND TECHNOLOGY (博士毕业论文) Cytokine-CpG Motif Oligodeoxynucleotide Co-inoculation in the Murine Malaria Model handle:123456789
Molecular Neurobiology Reduced Sympathetic Innervation in Endometriosis is Associated to Semaphorin 3C and 3FExpression. pubmed:27558236
Scand J Clin Lab Invest Serum concentrations of neuropilin-1 in women with endometriosis Pubmed: 32069143
Life Sciences Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817
Scientific Reports SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.
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