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Active Epithelial Neutrophil Activating Peptide 78 (ENA78)

CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78

ACTIVITY TEST

Epithelial Neutrophil Activating Peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines. Full-length ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. ENA-78 is expressed by many immune cells, such as macrophages, eosinophils, as well as non-immune cells including mesothelial cells, and fibroblasts. Soluble ENA-78 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of ENA-78 on THP-1 the human monocytic cell line. Briefly, THP-1 cells were seeded into the upper chambers (150 ul cell suspension,106 cells/ml in RPMI 1640 with FBS free) and different concentrations of recombinant mouse ENA-78 diluted with serum free RPMI 1640 was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37 ℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted using Fluorescence Activating Cell Sorter. Result shows ENA-78 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×10) were shown in Figure 1. Statistical results of FACS were shown in Figure 2. The optimum chemotaxis of ENA-78 occurs at 1 ng/ml.

Figure 2. The chemotactic effect of ENA-78 on THP-1 cells

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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SCA860Mu CLIA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Chemiluminescent immunoassay for Antigen Detection.
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