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Active Acid Phosphatase 1 (ACP1)

HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase

ACTIVITY TEST

Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 ( assay buffer), initiated by addition 50 μl of 3 ug/ml ACP1 (diluted by assay buffer) to 50 µL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 μl of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA994Mu01 Recombinant Acid Phosphatase 1 (ACP1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA994Mu02 Recombinant Acid Phosphatase 1 (ACP1) Positive Control; Immunogen; SDS-PAGE; WB.
APA994Mu02 Active Acid Phosphatase 1 (ACP1) Cell culture; Activity Assays.