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Figure. SDS-PAGE
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Active Motility Related Protein (MRP1)
CD9; TSPAN29; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; P24; Tetraspanin 29; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3
- Product No.APB097Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 212
US$ 530
US$ 1060
US$ 3180
US$ 7950
For more details, please contact local distributors!
ACTIVITY TEST
Motility Related Protein (MRP1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. It can enable ATPase-coupled inorganic anion transmembrane transporter activity. Involved in anion transmembrane transport ; renal tubular secretion ; and response to toxic substance. It is an integral component of plasma membrane. Besides, ITGb1 has been identified as an interactor of MRP1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MRP1 and recombinant human ITGb1. Briefly, biotin-linked MRP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MRP1 and ITGb1 was shown in Figure 1, the EC50 for this effect is 0.049 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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