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Figure. SDS-PAGE
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Instruction Manual
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Active Cluster Of Differentiation 8a (CD8a)
p32; CD8-A; Leu2; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
- Product No.APB099Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point9.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 300
US$ 750
US$ 1500
US$ 4500
US$ 11250
For more details, please contact local distributors!
ACTIVITY TEST
Cluster Of Differentiation 8a (CD8a) is an approximately 32-34 kDa cell surface glycoprotein found on cytotoxic T lymphocytes. It belongs to the immunoglobulin family and is a key component of the immune system, particularly in the context of T-cell biology. Its interaction with MHC class I molecules is crucial for T-cell development, activation, and the mediation of the immune response against infected or cancerous cells.CD4 and CD8 molecules play a key role in the interaction between T cells and antigen presenting cells (APCs), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD8a and recombinant human CD4. Briefly, biotin-linked CD8a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD8a and recombinant human CD4 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494 |