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Active Hyaluronidase (HAase)

Hyaluronic Lase; Hyaluroniclase

ACTIVITY TEST

Hyaluronidase (HAase) is a general term for enzymes that can hydrolyze hyaluronic acid. It is an enzyme that can reduce the activity of hyaluronic acid in the body, thereby improving the fluid permeability in tissues. When used in the human body, it can temporarily reduce the viscosity of the intercellular matrix, promote subcutaneous infusion, locally accumulated exudate or blood to accelerate diffusion and facilitate absorption, and is an important drug dispersant. Clinically used as a drug penetrating agent to promote drug absorption, promote local edema or hematoma dissipation after surgery and trauma. Human HAase consists of 435 amino acids which contains a signal peptide of 1-21 amino acids and it shares 74% and 75% amino acid sequence homology with mouse and rat respectively. The activity assay of recombinant human HAase was measured by its ability to hydrolyze the substrate hyaluronic acid. The rhHAase was diluted to 0.5 ug/ml in 8.46 mM NaH2PO4, 11.54 mM Na2HPO4, 77 mM NaCl, 0.1 mg/ml BSA, pH 7. 50 ul 0.5 ug/ml rhHAase was added into the microplate and start the reaction by adding 50 µL of 0.3 mg/ml substrate which was diluted in 300mM NaH2PO4, pH 5.35. Incubated at 37 °C for 5min and add 50 ul reaction mixture to 250 ul 1 mg/ml BSA in 24 mM sodium acetic, 79 mM acetic acid, pH 3.75. Incubated at room temperature for 10min and read at a wavelength of 600 nm. The specific activity of recombinant human HAase is >30 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Research in Pharmacy and Science Pepsin, Papain and Hyaluronidase Enzyme Analysis: A Review. Ebscohost: Source
Diabetes Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes Pubmed:29986925
Critical Care 6% Hydroxyethyl starch (HES 130/0.4) diminishes glycocalyx degradation and decreases vascular permeability during systemic and pulmonary inflammation … Pubmed:29716625
Pakistan Journal of Pharmaceutical Sciences Hyaluronan as a mediator for the hepatoprotective effect of Diosmin/Hesperidin complex. Pubmed:30033401
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB217Hu61 Active Hyaluronidase (HAase) Cell culture; Activity Assays.
RPB217Hu01 Recombinant Hyaluronidase (HAase) Positive Control; Immunogen; SDS-PAGE; WB.
RPB217Hu02 Recombinant Hyaluronidase (HAase) Positive Control; Immunogen; SDS-PAGE; WB.
PAB217Hu01 Polyclonal Antibody to Hyaluronidase (HAase) WB; IHC; ICC; IP.
SEB217Hu ELISA Kit for Hyaluronidase (HAase) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB217Hu CLIA Kit for Hyaluronidase (HAase) Chemiluminescent immunoassay for Antigen Detection.
LMB217Hu Multiplex Assay Kit for Hyaluronidase (HAase) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.