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Active Integrin Alpha 2 (ITGa2)

CD49b; BR; GPIa; VLA-2; VLAA2; Platelet membrane glycoprotein Ia; Alpha 2 Subunit Of VLA-2 Receptor; Very Late Activation Proteins 2 Receptor; Collagen receptor; VLA-2 alpha

ACTIVITY TEST

Integrin Alpha 2 (ITGa2), also known as CD49b, is one of twelve integrin family alpha subunits which is pivotal to sustain the integrity of the cytoskeletal–extracellular matrix linkage among all cell adhesion receptors. Some research had shown that ITGa2 is a cell transmembrane receptor that assists the adhesion of other several cells to the extracellular matrix. In addition, changes of ITGa2 expression affect the immune microenvironment and immunogenicity of tumors. Integrin Beta 1 (ITGb1) has been identified as an interactor of ITGa2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse ITGa2 and recombinant mouse ITGb1. Briefly, ITGa2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ITGa2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse ITGa2 and recombinant mouse ITGb1 was shown in Figure 1, the EC50 for this effect is 0.6 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Biol Chem. Integrin αvβ1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-Mediated Cell-Cell Fusion and Virus Infection Pubmed:27226547
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APB228Mu01 Active Integrin Alpha 2 (ITGa2) Cell culture; Activity Assays.
RPB228Mu01 Recombinant Integrin Alpha 2 (ITGa2) Positive Control; Immunogen; SDS-PAGE; WB.
PAB228Mu01 Polyclonal Antibody to Integrin Alpha 2 (ITGa2) WB; IHC; ICC; IP.