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Active Elastin (ELN)

WBS, WS, SVAS; Tropoelastin; Supravalvular Aortic Stenosis; Williams-Beuren Syndrome

ACTIVITY TEST

Elastin (ELN) is an extracellular matrix (ECM) protein responsible for the extensibility and elastic recoil of many vertebrate tissues. ELN is abundant in elastic tissues, and tissues rich in elastin include the aorta and major blood vessels, the lungs, elastic ligaments, tendons and the skin. ELN can bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS), autosomal dominant cutis laxa. Decorin (DCN), an extracellular matrix (ECM) protein, is one of targets of ECM. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse ELN and recombinant human DCN. Briefly, ELN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DCN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ELN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse ELN and recombinant human DCN was shown in Figure 1, the EC50 for this effect is 0.068 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB337Mu01 Recombinant Elastin (ELN) Positive Control; Immunogen; SDS-PAGE; WB.
RPB337Mu02 Recombinant Elastin (ELN) Positive Control; Immunogen; SDS-PAGE; WB.
APB337Mu01 Active Elastin (ELN) Cell culture; Activity Assays.
PAB337Mu01 Polyclonal Antibody to Elastin (ELN) WB; IHC; ICC; IP.
PAB337Mu02 Polyclonal Antibody to Elastin (ELN) WB; IHC; ICC; IP.
LAB337Mu81 FITC-Linked Polyclonal Antibody to Elastin (ELN) WB; IHC; ICC; IF.
LAB337Mu71 Biotin-Linked Polyclonal Antibody to Elastin (ELN) WB; IHC; ICC.
SEB337Mu ELISA Kit for Elastin (ELN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB337Mu Multiplex Assay Kit for Elastin (ELN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.