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Active Defensin Beta 1 (DEFb1)

B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1

ACTIVITY TEST

Beta Defensin-1 (DEFB1) displays potent microcidal properties, and also plays a part in other aspects of innate and adaptive immunity. DEFB1 genetic variations have been reported as contributing to hBD1 production impairment, leading to a greater susceptibility to be infected by oral pathogens, also leading to periodontitis. To counteract host immunity, cryptosporidium parvum has evolved multiple strategies to suppress host antimicrobial defense. One such strategy is to reduce the production of the antimicrobial peptide beta-defensin 1 (DEFB1) by host epithelial cells. Beta-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity. It is reported that DEFb2 has been identified as an interactor of DEFb1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DEFb1 and recombinant human DEFb2. Briefly, DEFb2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DEFb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DEFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of DEFb1 and DEFb2 was shown in Figure 1. When recombinant human DEFb1 is lmmobilized at 2 ug/mL (100 uL/well), the concentration of rhDEFb2 that produces 50% optimal binding response is found to be approximately 0.11 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Appl Environ Microbiol. Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source
British Journal of Nutrition Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699
International Journal of Dairy Technology Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111
Molecular Nutrition Food Research Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948
Frontiers in Microbiology Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590
International Immunopharmacology Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347
cyberleninka EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Animals Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218
Sci Rep Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937
J Mol Histol Isolation, culture, and identification of ceruminous gland cells Pubmed:35113280
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB373Hu01 Active Defensin Beta 1 (DEFb1) Cell culture; Activity Assays.
RPB373Hu01 Recombinant Defensin Beta 1 (DEFb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB373Hu01 Polyclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
MAB373Hu22 Monoclonal Antibody to Defensin Beta 1 (DEFb1) WB; IHC; ICC; IP.
SEB373Hu ELISA Kit for Defensin Beta 1 (DEFb1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB373Hu CLIA Kit for Defensin Beta 1 (DEFb1) Chemiluminescent immunoassay for Antigen Detection.
LMB373Hu Multiplex Assay Kit for Defensin Beta 1 (DEFb1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.