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Active Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

ACTIVITY TEST

Calreticulin (CALR) is an endoplasmic reticulum (ER)-resident protein involved in a spectrum of cellular processes. In healthy cells, CALR operates as a chaperone and Ca2  buffer to assist correct protein folding within the ER. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type. Adhesive glycoprotein THBS1 has been shown to play roles in platelet aggregation, angiogenesis and tumorigenesis, the protein is an interactor of CALR, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CALR and recombinant rat THBS1. Briefly, biotin-linked CALR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to THBS1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CALR andTHBS1 was shown in Figure 1, the EC50 for this effect is 0.018 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Investigative Dermatology Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes Pubmed: 23771121
International Journal of Molecular Sciences The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Mdpi: Source
The Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes Pubmed:26953684
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. pubmed:28152632
Talanta Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling Doi: 10.1016/j.talanta.2018.11.098
Sci Adv Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia Pubmed:35171673
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