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Gene sequencing
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Figure. SDS-PAGE
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Instruction Manual
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Active Tubulin Beta (TUBb)
TUB-B; TUBB5; TUBB1; M40
- Product No.APB870Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 331
US$ 828
US$ 1656
US$ 4968
US$ 12420
For more details, please contact local distributors!
ACTIVITY TEST
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin Beta (TUBb) is a spherical molecule which is composed of 455 amino acids. Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) has a remarkably high affinity for TUBb, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human YWHAz. Briefly, TUBb was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to YWHAz-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human TUBb and recombinant human YWHAz was shown in Figure 1, the EC50 for this effect is 0.88 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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