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Active S100 Calcium Binding Protein A7 (S100A7)

S100-A7; PSOR1; S100A7c; Psoriasin 1

ACTIVITY TEST

S100 Calcium Binding Protein A7 (S100A7), a member of the S100 family of vertebrate proteins, is an EF-hand type calcium binding protein localized in epithelial cells. S100A7 regulates cell proliferation and differentiation. An S100A7 overexpression may occur in response to inflammatory stimuli, such in psoriasis, besides S100A7 expression is upregulated in several cancers including skin, breast, lung, head, neck, cervix, bladder and gastric cancer. S100 Calcium Binding Protein A12 (S100A12) has been identified as an interactor of S100A7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human S100A7 and recombinant pig S100A12. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A12-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human S100A7 and recombinant pig S100A12 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Archives of Dermatological Research Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters SpringerLink: t07u24t43r357l15
J Proteome Res Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach PubMed: 25807139
Exp Dermatol S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling Pubmed:27060579
Experimental Dermatology S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signalling pubmed:27060579
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription pubmed:28672924
Scientific Reports The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function. pubmed:29142248
Journal of the European Academy of Dermatology and Venereology A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis Pubmed:28681537
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC035Hu01 Recombinant S100 Calcium Binding Protein A7 (S100A7) Positive Control; Immunogen; SDS-PAGE; WB.
APC035Hu01 Active S100 Calcium Binding Protein A7 (S100A7) Cell culture; Activity Assays.
PAC035Hu01 Polyclonal Antibody to S100 Calcium Binding Protein A7 (S100A7) WB
LAC035Hu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A7 (S100A7) WB; IHC; ICC.
MAC035Hu22 Monoclonal Antibody to S100 Calcium Binding Protein A7 (S100A7) WB; IHC; ICC; IP.
SEC035Hu ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC035Hu Multiplex Assay Kit for S100 Calcium Binding Protein A7 (S100A7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.