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Active Bone Morphogenetic Protein 8B (BMP8B)

BMP8-B; OP2; Osteogenic Protein 2

ACTIVITY TEST

Bone Morphogenetic Protein 8B (BMP8B) is a protein that belongs to the bone morphogenetic protein family, which is involved in the regulation of growth and differentiation of various cell types, including osteoblasts, chondrocytes, and neurons. It plays a role in skeletal development and tissue repair processes.Besides,Bone Morphogenetic Protein 8A (BMP8A) has been identified as an interactor of BMP8B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse BMP8B and recombinant human BMP8A. Briefly, a functional ELISA assay was conducted to detect the interaction of recombinant mouse BMP8B and recombinant human BMP8A. Briefly, BMP8B was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP8A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BMP8B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse BMP8B and recombinant human BMP8A was shown in Figure 1, the EC50 for this effect is 0.078ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC105Mu01 Recombinant Bone Morphogenetic Protein 8B (BMP8B) Positive Control; Immunogen; SDS-PAGE; WB.
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