≡

Active Amine Oxidase, Copper Containing 3 (AOC3)

VAP1; HPAO; SSAO; Membrane primary amine oxidase; Vascular Adhesion Protein 1; Semicarbazide-Sensitive Amine Oxidase; Copper-Containing Amine Oxygen Oxidoreductase

ACTIVITY TEST

Amine Oxidase, Copper Containing 3 (AOC3) is a copper amine oxidase with a topaquinone cofactor. AOC3 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. AOC3 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. The enzyme is sensitive to inhibition by semicarbazide. AOC3 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver. The activity of recombinant mouse AOC3 was measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of AOC3 (diluted by assay buffer) to 50 µl substrate mixture of 2 mM Benzylamine, 2 units/mL HRP and 100 µM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APC298Mu61 Active Amine Oxidase, Copper Containing 3 (AOC3) Cell culture; Activity Assays.
RPC298Mu01 Recombinant Amine Oxidase, Copper Containing 3 (AOC3) Positive Control; Immunogen; SDS-PAGE; WB.
EPC298Mu61 Eukaryotic Amine Oxidase, Copper Containing 3 (AOC3) Positive Control; Immunogen; SDS-PAGE; WB.
RPC298Mu02 Recombinant Amine Oxidase, Copper Containing 3 (AOC3) Positive Control; Immunogen; SDS-PAGE; WB.
PAC298Mu02 Polyclonal Antibody to Amine Oxidase, Copper Containing 3 (AOC3) WB; IHC; ICC; IP.
PAC298Mu01 Polyclonal Antibody to Amine Oxidase, Copper Containing 3 (AOC3) WB; IHC; ICC; IP.
SEC298Mu ELISA Kit for Amine Oxidase, Copper Containing 3 (AOC3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC298Mu Multiplex Assay Kit for Amine Oxidase, Copper Containing 3 (AOC3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.