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Active Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2)

CPTASE; Carnitine O-Palmitoyltransferase 2; Carnitine palmitoyltransferase II

ACTIVITY TEST

Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2), member of the carnitine shuttle, is involved in the transport of long-chain fatty acids into the mitochondrial compartment.CPT2 is located in the inner mitochondrial membrane, and its deficiency is an inherited metabolic disease.Three forms of CPT2 deficiency have been described: a myopathic form, a severe infantile form and a neonatal form.Acyl Coenzyme A Dehydrogenase, Short/Very Long Chain (ACADVL) is active toward esters of long-chain and very long chain fatty acids, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CPT2 and recombinant human ACADVL.Briefly, biotin-linked CPT2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ACADVL-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant mouse CPT2 and recombinant human ACADVL was shown in Figure 1, the EC50 for this effect is 0.08ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of the Neurological Sciences Normal protein content but abnormally inhibited enzyme activity in muscle carnitine palmitoyltransferase II deficiency ScienceDirect: S0022510X14000975
World journal of gastroenterology Mitochondrial carnitine palmitoyl transferase-II inactivity aggravates lipid accumulation in rat hepatocarcinogenesis. pubmed:28127199
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APC417Mu01 Active Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Cell culture; Activity Assays.
RPC417Mu01 Recombinant Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Positive Control; Immunogen; SDS-PAGE; WB.
SEC417Mu ELISA Kit for Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Enzyme-linked immunosorbent assay for Antigen Detection.