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Active Catalase (CAT)

ACTIVITY TEST

Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable yellow product which has a absorbance at 405 nm. Thus, the recombinant human CAT activity was measured by its ability to hydrolyze H2O2 to H2O , the remaining H2O2 in the reaction was measured by (NH4)2MoO4. The reaction was performed in 60 mM phosphate buffer pH 7.4 (Assay Buffer), initiated by addition 100 μL of various concentrations of CAT (diluted by Assay Buffer) to 1 mL of 65 mM Substrate H2O2 (diluted by Assay Buffer). Incubated at 37℃ for 1min, then add 1 ml 200 mM (NH4)2MoO4. After 10 minutes at room temperature, read at a wavelength of 405 nm.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Arteriosclerosis, Thrombosis, and Vascular Biology Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm Ahajournals: source
Fundam Clin Pharmacol. Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activities PubMed: 20880383
Oral Disease Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats Pubmed: 24106991
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. Pubmed: 24142649
Journal of Food Science and Technology Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats Springer: Source
第 三 军 医 大 学 学 报 过氧化氢酶基因 ３８９Ｃ＞Ｔ多态位点在重庆汉族人群中的分布及其与噪音性耳聋的 关联研究 Tmmu:Source
Adv Med Sci Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
Arch Oral Biol Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes PubMed: 26143097
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ docs:225188
Current Alzheimer Research Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. pubmed:27748187
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines jcrt:5
Adv Med Sci. Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. pubmed:28242484
PeerJ Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2 pubmed:28785522
Pharmacological Reports Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder. pubmed:28554099
Ecotoxicology and Environmental Safety Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil Pubmed:29660615
Human and Ecological Risk Assessment: An International Journal Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails 10.1080:10807039.2018.1460800
Experimental and Therapeutic Medicine Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Pubmed:30116359
Journal of Cosmetic Dermatology A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling Pubmed:30047218
Anticancer Research Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different … Pubmed: 30194197
Anticancer Research Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with Cancer Pubmed: 30396975
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Doi: 10.1016/j.joim.2018.12.002
EXPERIMENTAL ANIMALS Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation Pubmed: 30606939
MOLECULAR BIOLOGY REPORTS An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cells Pubmed: 30628023
Anticancer Research Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients Pubmed: 30711961
Anticancer Research Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A … Pubmed: 30842172
Molecular Medicine Reports MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury Pubmed: 31115556
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance Pubmed: 31146169
European Journal of Biomedical and Pharmaceutical Sciences European Journal of Biomedical AND Pharmaceutical sciences
Anticancer Research The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer Pubmed: 31892574
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Biomolecules Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development Pubmed: 32751983
AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
The Journal of Physiology In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
Journal of Cell Communication and Signaling Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE …
Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic …
Malaysian Journal of Medicine and Health Sciences Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
nature environment pollution technology Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses
World Neurosurg Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­ 33689849
Biomed Res Int Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris 33763469
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Molecular Medicine Reports Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats 34278476
J Pers Med Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis 34945751
In Vivo Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer Patients Pubmed:35241533
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