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Figure. SDS-PAGE
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Instruction Manual
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Active Histidine Rich Glycoprotein (HRG)
HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG
- Product No.APC534Mu61
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point7.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 296
US$ 740
US$ 1480
US$ 4440
US$ 11100
For more details, please contact local distributors!
ACTIVITY TEST
Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant mouse HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT 4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat mouse HRG coated plates (different concentrations with 100 µL/well), cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MOLT-4 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in figure 1. The result of cell adhesion was shown in figure 2, the EC50 was 0.36 ug/ml.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z |
| Medical Oncology | Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer Springer: Source |
| Int J Cancer | The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein PubMed: 25363753 |
| Nucleic Acid Therapeutics | Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice Pubmed:27031383 |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html |
| Hypertension Research | Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia Pubmed:29416108 |
| journal of histochemistry & cytochemistry | Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase Pubmed: 31880188 |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … Pubmed: 32342301 |