≡

Active Microfibrillar Associated Protein 2 (MFAP2)

MAGP1; Microfibril-associated glycoprotein 1

ACTIVITY TEST

Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC618Hu01 Recombinant Microfibrillar Associated Protein 2 (MFAP2) Positive Control; Immunogen; SDS-PAGE; WB.
APC618Hu01 Active Microfibrillar Associated Protein 2 (MFAP2) Cell culture; Activity Assays.
PAC618Hu01 Polyclonal Antibody to Microfibrillar Associated Protein 2 (MFAP2) WB; IHC; ICC; IP.