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Active Fibroblast Growth Factor 3 (FGF3)

HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2

ACTIVITY TEST

Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR4. Briefly, biotin-linked FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF3 and recombinant human FGFR4 was shown in Figure 1, the EC50 for this effect is 0.52 ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Mol Med Rep Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library PubMed: 26323695
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC906Hu01 Recombinant Fibroblast Growth Factor 3 (FGF3) Positive Control; Immunogen; SDS-PAGE; WB.
APC906Hu01 Active Fibroblast Growth Factor 3 (FGF3) Cell culture; Activity Assays.
RPC906Hu02 Recombinant Fibroblast Growth Factor 3 (FGF3) Positive Control; Immunogen; SDS-PAGE; WB.
PAC906Hu01 Polyclonal Antibody to Fibroblast Growth Factor 3 (FGF3) WB; IHC; ICC; IP.
LAC906Hu71 Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 3 (FGF3) WB; IHC; ICC.
MAC906Hu22 Monoclonal Antibody to Fibroblast Growth Factor 3 (FGF3) WB; IHC; ICC; IP.
SEC906Hu ELISA Kit for Fibroblast Growth Factor 3 (FGF3) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC906Hu CLIA Kit for Fibroblast Growth Factor 3 (FGF3) Chemiluminescent immunoassay for Antigen Detection.
LMC906Hu Multiplex Assay Kit for Fibroblast Growth Factor 3 (FGF3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.