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Active Fibroblast Growth Factor 8, Androgen Induced (FGF8)

AIGF; HBGF8; Androgen-induced growth factor; Heparin-binding growth factor 8

ACTIVITY TEST

FGF8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Alternate splicing of mouse FGF8 mRNA generates eight secreted isoforms, designated a - h. Only FGF-8a, b, e and f exist in humans. FGF8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis. FGFR2c, 3c and FGFR4 can be activated by several FGF8 isoforms, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF8 and recombinant human FGFR4. Briefly, biotin-linked FGF8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of FGF-8 and FGFR4 was shown in Figure 1, the EC50 for this effect is 7.94 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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University of Pittsburgh Strategies to Target Estrogen-Related Tumor Promoting Mechanisms in Lung Cancer 0000-0001-5673-022X
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC908Hu02 Recombinant Fibroblast Growth Factor 8, Androgen Induced (FGF8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC908Hu03 Recombinant Fibroblast Growth Factor 8, Androgen Induced (FGF8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC908Hu01 Recombinant Fibroblast Growth Factor 8, Androgen Induced (FGF8) Positive Control; Immunogen; SDS-PAGE; WB.
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PAC908Hu01 Polyclonal Antibody to Fibroblast Growth Factor 8, Androgen Induced (FGF8) WB; IHC; ICC; IP.
PAC908Hu08 Polyclonal Antibody to Fibroblast Growth Factor 8, Androgen Induced (FGF8) WB; IHC; ICC; IP.
MAC908Hu21 Monoclonal Antibody to Fibroblast Growth Factor 8, Androgen Induced (FGF8) WB; IHC; ICC; IP.
SEC908Hu ELISA Kit for Fibroblast Growth Factor 8, Androgen Induced (FGF8) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC908Hu Multiplex Assay Kit for Fibroblast Growth Factor 8, Androgen Induced (FGF8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.