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Active Platelet Derived Growth Factor D (PDGFD)

PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form

ACTIVITY TEST

Platelet-derived growth factor D (PDGFD) is a protein that in humans is encoded by the PDGFD gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. PDGF plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis. PDGF also plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. Besides, Macrophage Erythroblast Attacher (MAEA) has been identified as an interactor of PDGFD, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFD and recombinant human MAEA. Briefly, PDGFD were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to MAEA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PDGFD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were
incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFD and MAEA was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of PDGFD with MAEA.

Figure. Western Blot

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Marmara Pharmaceutical Journal In vitro gene silencing effect of chitosan/shRNA PDGF-D nanoparticles in breast cancer DOI: 10.12991/mpj.2017.21
Cell Reports Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue Pubmed: 30332637
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC919Hu01 Recombinant Platelet Derived Growth Factor D (PDGFD) Positive Control; Immunogen; SDS-PAGE; WB.
RPC919Hu02 Recombinant Platelet Derived Growth Factor D (PDGFD) Positive Control; Immunogen; SDS-PAGE; WB.
RPC919Hu03 Recombinant Platelet Derived Growth Factor D (PDGFD) Positive Control; Immunogen; SDS-PAGE; WB.
EPC919Hu61 Eukaryotic Platelet Derived Growth Factor D (PDGFD) Positive Control; Immunogen; SDS-PAGE; WB.
APC919Hu01 Active Platelet Derived Growth Factor D (PDGFD) Cell culture; Activity Assays.
PAC919Hu01 Polyclonal Antibody to Platelet Derived Growth Factor D (PDGFD) WB; IHC; ICC; IP.
MAC919Hu22 Monoclonal Antibody to Platelet Derived Growth Factor D (PDGFD) WB; IHC; ICC; IP.
SEC919Hu ELISA Kit for Platelet Derived Growth Factor D (PDGFD) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC919Hu CLIA Kit for Platelet Derived Growth Factor D (PDGFD) Chemiluminescent immunoassay for Antigen Detection.
LMC919Hu Multiplex Assay Kit for Platelet Derived Growth Factor D (PDGFD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.