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Active Troponin T Type 2, Cardiac (TNNT2)
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T
- Product No.APD232Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point4.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 331
US$ 828
US$ 1656
US$ 4968
US$ 12420
For more details, please contact local distributors!
ACTIVITY TEST
Troponin T Type 2, Cardiac (TNNT2) is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury. Besides, Troponin I Type 3, Cardiac (TNNI3) has been identified as an interactor of TNNT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNT2 and recombinant human TNNTI3. Briefly, TNNT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TNNI3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNNT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of TNNT2 and TNNI3 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats PubMed: 21232020 |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats Wiley: source |
| European Journal of Pharmacology | Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats ScienceDirect: S0014299909009984 |
| Journal of translational medicine | The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury PubMed: PMC3694448 |
| Oral Diseases | Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction Pubmed: 22834943 |
| An International Journal of Pharmaceutical Sciences | In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium Pubmed: 23700796 |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source |
| Liver Int. | Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis Pubmed:24330504 |
| Cardiovasc Toxicol. | Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs Pubmed:24420420 |
| British Journal of Pharmacology | Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise Pubmed:25039894 |
| Chemico-Biological Interactions | Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765 |
| The Journal of Pharmacology and Experimental Therapeuties | The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice Pubmed:27247000 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. pubmed:27937003 |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155 |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830 |
| PLoS One | Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model pubmed:28570599 |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus pubmed:29042932 |
| PLoS One | Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury Pubmed: 30192780 |
| PLoS One | Tissue suction-mediated gene transfer to the beating heart in mice Pubmed: 32027678 |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy Pubmed: 32148580 |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535 |
| Toxics | Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats Pubmed: 32947820 |
| Elife | Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice 33526170 |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344 |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993 |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584 |