≡

Active Spleen Tyrosine Kinase (SYK)

p72-Syk; Spleen tyrosine kinase

ACTIVITY TEST

Figure. The binding activity of SYK with SIGLEC2.
Spleen Tyrosine Kinase (SYK) is a member of the SYK family of tyrosine kinases. These non-receptor cytoplasmic tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. Within B and T cells respectively, SYK and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. SYK plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. Besides, Sialic Acid Binding Ig Like Lectin 1 (SIGLEC2) has been identified as an interactor of SYK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SYK and recombinant human SIGLEC2. Briefly, SYK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to SIGLEC2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SYK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SYK and SIGLEC2 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Scientific Reports Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase Pubmed: 31949205
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE275Hu01 Recombinant Spleen Tyrosine Kinase (SYK) Positive Control; Immunogen; SDS-PAGE; WB.
APE275Hu01 Active Spleen Tyrosine Kinase (SYK) Cell culture; Activity Assays.
PAE275Hu01 Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) WB,IHC
MAE275Hu21 Monoclonal Antibody to Spleen Tyrosine Kinase (SYK) WB; IHC; ICC; IP.
SEE275Hu ELISA Kit for Spleen Tyrosine Kinase (SYK) Enzyme-linked immunosorbent assay for Antigen Detection.
LME275Hu Multiplex Assay Kit for Spleen Tyrosine Kinase (SYK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.