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Active Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

ACTIVITY TEST

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. To test the effect of ANXA1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant rat ANXA1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2h at 37oC. Proliferation of A549 cells after incubation with ANXA1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant rat ANXA1 for 48h. The result was shown in Figure 2. It was obvious that ANXA1 significantly inhibit cell viability of A549 cells. The ED50 is 1.75µg/mL.

Figure 2. Inhibition of A549 cells proliferation after stimulated with ANXA1.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Plos one Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease PubMed: PMC3794972
African Journals Online Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 Ajol: Source
Antimicrobial Agents and Chemotherapy Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response Asm: Source
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731
Diabetes Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function PubMed: 26470781
PLoS One Annexin A1 Is Increased in the Plasma of Preeclamptic Women PubMed: 26398190
中国现代医学杂志 China Journal of Modern Medicine 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义 article:8686
arteriosclerosis thrombosis and vascular biology Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. pubmed:28062503
Clinical Science Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. pubmed:29101297
Biomedicine & Pharmacotherapy Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity Pubmed:29677533
 International Journal of Chronic Obstructive Pulmonary Disease Annexin A1 is elevated in patients with COPD and affects lung fibroblast function Pubmed:29440885
Medical Mycology Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients Pubmed:29370415
DEVELOPMENTAL NEUROPSYCHOLOGY EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT … pdf:3395
Multiple sclerosis and related disorders Resolution of inflammation in neuromyelitis optica spectrum disorders Pubmed: 30300851
Acta Pharmaceutica Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
npj Science of Food Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites Pubmed: 32133417
FASEB JOURNAL ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway Pubmed: 32908567
Rev Soc Bras Med Trop Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients 33263684
science advances Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy 33523920
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPE787Ra02 Recombinant Annexin A1 (ANXA1) Positive Control; Immunogen; SDS-PAGE; WB.
RPE787Ra01 Recombinant Annexin A1 (ANXA1) Positive Control; Immunogen; SDS-PAGE; WB.
APE787Ra01 Active Annexin A1 (ANXA1) Cell culture; Activity Assays.
PAE787Ra01 Polyclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
LAE787Ra71 Biotin-Linked Polyclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC.
MAE787Ra27 Monoclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
MAE787Ra21 Monoclonal Antibody to Annexin A1 (ANXA1) WB,IHC
MAE787Ra22 Monoclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
MAE787Ra23 Monoclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
MAE787Ra24 Monoclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
MAE787Ra25 Monoclonal Antibody to Annexin A1 (ANXA1) WB; IHC; ICC; IP.
FAE787Ra04 Anti-Annexin A1 (ANXA1) Monoclonal Antibody Flow cytometry.
FAE787Ra02 Anti-Annexin A1 (ANXA1) Monoclonal Antibody Flow cytometry.
SEE787Ra ELISA Kit for Annexin A1 (ANXA1) Enzyme-linked immunosorbent assay for Antigen Detection.
LME787Ra Multiplex Assay Kit for Annexin A1 (ANXA1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSE787Ra01 ELISA Kit DIY Materials for Annexin A1 (ANXA1) Main materials for "Do It(ELISA Kit) Yourself"