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Active Carboxylesterase 2 (CES2)

CE-2; CES2A1; PCE-2; iCE; Intestine,Liver; Methylumbelliferyl-acetate deacetylase 2; Cocaine esterase

ACTIVITY TEST

Carboxylesterase 2 (CES2) is a member of serine esterase family which can hydrolyze ester and amide bonds. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES2 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, 150 mM NaCl, pH 7.5 (Assay Buffer), ainitiated by addition 50 μL of various concentrations of CES2 (diluted by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by Assay Buffer). Incubated at 37℃ for 10 min, then read at a wavelength of 400 nm.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Metallomics Comprehensive and comparative exploration of the Atp7b−/− mouse plasma proteome Pubmed: 31815268
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF282Hu01 Recombinant Carboxylesterase 2 (CES2) Positive Control; Immunogen; SDS-PAGE; WB.
APF282Hu61 Active Carboxylesterase 2 (CES2) Cell culture; Activity Assays.
EPF282Hu61 Eukaryotic Carboxylesterase 2 (CES2) Positive Control; Immunogen; SDS-PAGE; WB.
WEF282Hu Wide-range ELISA Kit for Carboxylesterase 2 (CES2) Enzyme-linked immunosorbent assay for Antigen Detection.
SEF282Hu ELISA Kit for Carboxylesterase 2 (CES2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF282Hu Multiplex Assay Kit for Carboxylesterase 2 (CES2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.