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Figure. SDS-PAGE
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Instruction Manual
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Active Lecithin Cholesterol Acyltransferase (LCAT)
phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
- Product No.APJ516Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
LCAT(Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines(lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that Apolipoprotein E (apoE) is the main LCAT activator in glia- conditioned media (GCM). Thus, a binding ELISA assay was conducted to detect the association of recombinant human LCAT with recombinant human apoE. Briefly, recombinant human LCAT were diluted serially in PBS with 0.01% BSA(pH 7.4). Duplicate samples of 100ul were then transferred to apoE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with apoE was shown in Figure 1 ,the EC50 for this effect is 0.036ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Inflammation. | Alteration of Lysophosphatidylcholine-Related Metabolic Parameters in the Plasma of Mice withExperimental Sepsis. pubmed:28028754 |
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| British Journal of Cancer | Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel. Pubmed: 31388184 |
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| Cell Metab | Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression Pubmed:35235775 |