≡

Active MHC Class I Polypeptide Related Sequence A (MICA)

PERB11.1

ACTIVITY TEST

MHC Class I Polypeptide Related Sequence A (MICA) is a stress-induced cell surface protein. It belongs to the MHC class I-like molecules but has distinct features. MICA is highly expressed on cells under stress, such as those with DNA damage or infected cells. Structurally, it has a domain structure that allows for interactions with other molecules.MICA can function as a ligand and binds to Killer Cell Lectin Like Receptor Subfamily D, Member 1 (KLRD1, also known as NKG2D) on the surface of natural killer (NK) cells and some T cells. This binding activates immune cells and enables them to participate in the body's immune surveillance and defense responses.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human MICA and recombinant human KLRD1. Briefly, MICA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to KLRD1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MICA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human MICA and recombinant human KLRD1 was shown in Figure 1, the EC50 for this effect is 0.582ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APP871Hu01 Active MHC Class I Polypeptide Related Sequence A (MICA) Cell culture; Activity Assays.
RPP871Hu01 Recombinant MHC Class I Polypeptide Related Sequence A (MICA) Positive Control; Immunogen; SDS-PAGE; WB.
MAP871Hu21 Monoclonal Antibody to MHC Class I Polypeptide Related Sequence A (MICA) WB; IHC; ICC; IP.
SEP871Hu ELISA Kit for MHC Class I Polypeptide Related Sequence A (MICA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMP871Hu Multiplex Assay Kit for MHC Class I Polypeptide Related Sequence A (MICA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.