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CLIA Kit for Free Thyroxine (fT4)
FTI
- Product No.CCA185Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range781.25-200,000fg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 272.41fg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 514
US$ 734
US$ 3303
US$ 6239
US$ 51380
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Free Thyroxine (fT4).
No significant cross-reactivity or interference between Free Thyroxine (fT4) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Free Thyroxine (fT4) and the recovery rates were calculated by comparing the measured value to the expected amount of Free Thyroxine (fT4) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-101 | 83 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 93-103 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Free Thyroxine (fT4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Free Thyroxine (fT4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Free Thyroxine (fT4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-91% | 81-102% | 87-95% | 78-93% |
| EDTA plasma(n=5) | 81-92% | 78-101% | 83-93% | 82-95% |
| heparin plasma(n=5) | 97-105% | 79-98% | 99-105% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Substrate B | 1×2mL |
| Substrate A | 1×10mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
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| Magazine | Citations |
| Frontiers in cellular neuroscience | Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice pubmed:28119573 |
| ARC Journal of Nutrition and Growth | Effect of Selenium and Zinc Supplementation on Hypothyroidism in Rats ISSN 2455-2550 |
| Frontiers in Neuroscience | Modulation of Calcitonin, Parathyroid Hormone, and Thyroid Hormone Secretion by Electrical Stimulation of Sympathetic and Parasympathetic Nerves in Anesthetized Rats. pubmed:28713236 |
| Brain Research | Age-Dependent Evaluation of Long-Term Depression Responses in Hyperthyroid Rats: Possible Roles of Oxidative Intracellular Redox Status Pubmed: 31288003 |
| Journal of Physiological Sciences | Thyroxin and calcitonin secretion into thyroid venous blood is regulated by pharyngeal mechanical stimulation in anesthetized rats Pubmed: 31270742 |
| International Journal of Clinical and Experimental Medicine | Apatinib inhibits proliferation of different lung cancer cells in vitro and in vivo by promoting apoptosis and inducing cell cycle arrest |
| Int J Dev Neurosci | Effect of L©\thyroxine administration on long©\term potentiation and accompanying mitogen©\activated protein kinases in rats 33576121 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| IEA185Ge | Instant ELISA Kit for Free Thyroxine (fT4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA185Ge | ELISA Kit for Free Thyroxine (fT4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA185Ge | CLIA Kit for Free Thyroxine (fT4) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA185Ge | Multiplex Assay Kit for Free Thyroxine (fT4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |