Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Maybe you can also pay attention to our smart microplate reader
ELISA Kit for C-Peptide (CP)
- Product No.CEA447Si
- Organism SpeciesRhesus monkey (Simian) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 53.5pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 567
US$ 810
US$ 3645
US$ 6885
US$ 56700
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of C-Peptide (CP).
No significant cross-reactivity or interference between C-Peptide (CP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant C-Peptide (CP) and the recovery rates were calculated by comparing the measured value to the expected amount of C-Peptide (CP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-94 | 86 |
| EDTA plasma(n=5) | 90-99 | 93 |
| heparin plasma(n=5) | 87-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Peptide (CP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide (CP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-101% | 88-96% | 93-102% | 93-105% |
| EDTA plasma(n=5) | 98-105% | 93-101% | 93-101% | 85-101% |
| heparin plasma(n=5) | 91-98% | 78-96% | 93-101% | 90-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Journal of Animal Science | The effect of oral and intravenous dextrose on C-peptide secretion in ponies Pubmed:27065127 |
| Medical Science Monitor | Increased β-Cell Mass in Obese Rats after Gastric Bypass: A Potential Mechanism for Improving Glycemic Control. pubmed:28477035 |
| Journal of Alzheimers Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720 |
| Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in Pubmed: 31411368 |
| Journal of dermatological treatment | Liraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skin Pubmed: 31868553 |
| Journal of Diabetes Research | Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway Pubmed: 32309446 |
| Brain Research | Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway Pubmed: 32304687 |
| Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer 33602229 |
| EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats |
| Scientific Reports | SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. |
| Catalog No. | Related products for research use of Rhesus monkey (Simian) Organism species | Applications (RESEARCH USE ONLY!) |
| CEA447Si | ELISA Kit for C-Peptide (CP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA447Si | Multiplex Assay Kit for C-Peptide (CP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |