Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Maybe you can also pay attention to our smart microplate reader
ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)
NTX-I; NTX1
- Product No.CEA639Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.92ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
US$ 630
US$ 2835
US$ 5355
US$ 44100
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Cross Linked N-Telopeptide Of Type I Collagen (NTXI).
No significant cross-reactivity or interference between Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-101 | 89 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 78-98 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 95-103% | 88-97% | 85-94% |
| EDTA plasma(n=5) | 95-102% | 84-93% | 98-105% | 87-104% |
| heparin plasma(n=5) | 88-99% | 90-98% | 98-105% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity ScienceDirect: S0899900709004717 |
| Bone | Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca diet ScienceDirect: S875632820800776X |
| Journal of Orthopaedic Research | Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis model Wiley: Source |
| The Journal of Clinical Endocrinology & Metabolism | A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk PubMed: PMC3462931 |
| Acta Paediatr.? | Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolism Pubmed:25109232 |
| PLoS One. | An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice Pubmed:25184758 |
| J Bone Miner Res | Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis Imperfecta PubMed: 26679066 |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561 |
| Archives of Oral Biology | Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants. pubmed:27328151 |
| Journal of Periodontology | Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo … Pubmed:29520826 |
| Clinical oral implants research | Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis … Pubmed: 30659666 |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209 |
| Bioengineering (Basel) | Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two Cryogels Pubmed: 32517006 |
| BioMed Research International | Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta 33506016 |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043 |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780 |