≡

ELISA Kit for Pancreatic Polypeptide (PP)

PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone

Specificity

This assay has high sensitivity and excellent specificity for detection of Pancreatic Polypeptide (PP).
No significant cross-reactivity or interference between Pancreatic Polypeptide (PP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pancreatic Polypeptide (PP) and the recovery rates were calculated by comparing the measured value to the expected amount of Pancreatic Polypeptide (PP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 81-104 93
heparin plasma(n=5) 90-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pancreatic Polypeptide (PP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 94-102% 89-97% 82-99%
EDTA plasma(n=5) 90-98% 97-104% 91-101% 80-102%
heparin plasma(n=5) 92-101% 92-101% 86-104% 84-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB265Hu01 Recombinant Pancreatic Polypeptide (PP) Positive Control; Immunogen; SDS-PAGE; WB.
SPB265Hu01 Synthetic Pancreatic Polypeptide (PP) Immunogen; Blocking Peptide.
CPB265Hu21 OVA Conjugated Pancreatic Polypeptide (PP) Immunogen; SDS-PAGE; WB.
PAB265Hu01 Polyclonal Antibody to Pancreatic Polypeptide (PP) WB; IHC; ICC; IP.
MAB265Hu22 Monoclonal Antibody to Pancreatic Polypeptide (PP) WB; IHC; ICC; IP.
SEB265Hu ELISA Kit for Pancreatic Polypeptide (PP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB265Hu ELISA Kit for Pancreatic Polypeptide (PP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB265Hu Multiplex Assay Kit for Pancreatic Polypeptide (PP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB265Hu11 ELISA Kit DIY Materials for Pancreatic Polypeptide (PP) Main materials for "Do It (ELISA Kit) Yourself".