≡

ELISA Kit for Angiotensin III (AngIII)

Ang-III; Angiotensin-3

Specificity

This assay has high sensitivity and excellent specificity for detection of Angiotensin III (AngIII).
No significant cross-reactivity or interference between Angiotensin III (AngIII) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Angiotensin III (AngIII) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin III (AngIII) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-102 98
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 79-94 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin III (AngIII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin III (AngIII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin III (AngIII) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-104% 79-93% 93-101% 78-104%
EDTA plasma(n=5) 81-101% 84-92% 83-104% 91-98%
heparin plasma(n=5) 79-98% 94-104% 99-105% 85-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Mol Pain Mechanisms underlying clinical efficacy of Angiotensin II type 2 receptor (AT2R) antagonist EMA401 in neuropathic pain: clinical tissue and in vitro studies PubMed: 26111701
Journal of Atherosclerosis and Thrombosis Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro Pubmed:26947595
Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CEC312Ra ELISA Kit for Angiotensin III (AngIII) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC312Ra Multiplex Assay Kit for Angiotensin III (AngIII) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC312Ra11 ELISA Kit DIY Materials for Angiotensin III (AngIII) Main materials for "Do It (ELISA Kit) Yourself".