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ELISA Kit for Urocortin 3 (UCN3)

SCP; SPC; UCNIII; Stresscopin

Specificity

This assay has high sensitivity and excellent specificity for detection of Urocortin 3 (UCN3).
No significant cross-reactivity or interference between Urocortin 3 (UCN3) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Urocortin 3 (UCN3) and the recovery rates were calculated by comparing the measured value to the expected amount of Urocortin 3 (UCN3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 82-98 94
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin 3 (UCN3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin 3 (UCN3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Urocortin 3 (UCN3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-92% 90-104% 81-102% 82-92%
EDTA plasma(n=5) 91-101% 83-98% 80-93% 80-95%
heparin plasma(n=5) 78-98% 84-94% 83-96% 80-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD140Ra01 Recombinant Urocortin 3 (UCN3) Positive Control; Immunogen; SDS-PAGE; WB.
CPD140Ra31 KLH Conjugated Urocortin 3 (UCN3) Immunogen; SDS-PAGE; WB.
CPD140Ra21 OVA Conjugated Urocortin 3 (UCN3) Immunogen; SDS-PAGE; WB.
PAD140Ra08 Polyclonal Antibody to Urocortin 3 (UCN3) WB; IHC; ICC; IP.
PAD140Ra01 Polyclonal Antibody to Urocortin 3 (UCN3) WB; IHC; ICC; IP.
MAD140Ra21 Monoclonal Antibody to Urocortin 3 (UCN3) WB; IHC; ICC; IP.
CED140Ra ELISA Kit for Urocortin 3 (UCN3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD140Ra Multiplex Assay Kit for Urocortin 3 (UCN3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.