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High Sensitive ELISA Kit for Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- Product No.HEA693Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 485
US$ 693
US$ 3119
US$ 5891
US$ 48510
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Heat Shock Protein 27 (Hsp27).
No significant cross-reactivity or interference between High Sensitive Heat Shock Protein 27 (Hsp27) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Heat Shock Protein 27 (Hsp27) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Heat Shock Protein 27 (Hsp27) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-88 | 84 |
| EDTA plasma(n=5) | 80-92 | 84 |
| heparin plasma(n=5) | 82-94 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Heat Shock Protein 27 (Hsp27) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Heat Shock Protein 27 (Hsp27) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Heat Shock Protein 27 (Hsp27) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 80-98% | 78-103% | 90-97% |
| EDTA plasma(n=5) | 93-105% | 96-104% | 87-94% | 80-99% |
| heparin plasma(n=5) | 79-101% | 87-101% | 78-91% | 79-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells PubMed: 26467738 |
| Nature Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis Pubmed:29650953 |
| Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028 |
| Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity Pubmed: 31102684 |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort Pubmed: 32210109 |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771 |
| Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation |