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High Sensitive ELISA Kit for Alpha-Lactalbumin (aLA)
LALBA; LYZL7; Lactose Synthase B Protein; Lysozyme Like Protein 7
- Product No.HEB018Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum, plasma, milk, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.37ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 615
US$ 878
US$ 3951
US$ 7463
US$ 61460
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Alpha-Lactalbumin (aLA).
No significant cross-reactivity or interference between High Sensitive Alpha-Lactalbumin (aLA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Alpha-Lactalbumin (aLA) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Alpha-Lactalbumin (aLA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-102 | 99 |
| EDTA plasma(n=5) | 82-101 | 95 |
| heparin plasma(n=5) | 82-104 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Alpha-Lactalbumin (aLA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Alpha-Lactalbumin (aLA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Alpha-Lactalbumin (aLA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-93% | 89-99% | 82-95% | 97-105% |
| EDTA plasma(n=5) | 96-105% | 91-105% | 85-92% | 83-101% |
| heparin plasma(n=5) | 97-105% | 81-101% | 95-102% | 87-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| ALLERGY | Hypoallergenicity of a whey‐based, extensively hydrolyzed infant formula prepared with non‐porcine enzymes |
| Foods | Antigenicity and Safety Evaluation of Lactiplantibacillus plantarum 7-2 Screened to Reduce α-Casein Antigen Pubmed:35010214 |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093 |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB018Bo01 | Recombinant Alpha-Lactalbumin (aLA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB018Bo01 | Polyclonal Antibody to Alpha-Lactalbumin (aLA) | WB; IHC; ICC; IP. |
| MAB018Bo21 | Monoclonal Antibody to Alpha-Lactalbumin (aLA) | WB; IHC; ICC; IP. |
| HEB018Bo | High Sensitive ELISA Kit for Alpha-Lactalbumin (aLA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB018Bo | ELISA Kit for Alpha-Lactalbumin (aLA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB018Bo | Multiplex Assay Kit for Alpha-Lactalbumin (aLA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB018Bo01 | ELISA Kit DIY Materials for Alpha-Lactalbumin (aLA) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGB018Bo01 | Primer Pair for Alpha-Lactalbumin (aLA) | PCR |
| CGB018Bo01 | cDNA of Alpha-Lactalbumin (aLA) | PCR; Protein Expression; etc. |