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ELISA Kit DIY Materials for Angiogenin (ANG)

RNASE5; Ribonuclease,RNase A Family 5

  • Product No.KSA007Po01
  • Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.257ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1361  US$ 2268  US$ 4082  US$ 7938  US$ 13608 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Angiogenin (ANG). No significant cross-reactivity or interference between Angiogenin (ANG) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Inflammopharmacology Evening primrose oil and celecoxib inhibited pathological angiogenesis, inflammation, and oxidative stress in adjuvant-induced arthritis: novel role of angiopoietin-1 Pubmed: 24664592
Cancer Cell Angiogenin/Ribonuclease 5 is an EGFR ligand and a serum biomarker for erlotinib sensitivity in pancreatic cancer Pubmed:29606349
Journal of Immunotherapy The VE-PTP Inhibitor AKB-9778 Improves Antitumor Activity and Diminishes the Toxicity of Interleukin 2 (IL-2) Administration Pubmed: 31348125
Clinical Hemorheology and Microcirculation Nonselective alpha-/beta-AR antagonists can inhibit pericyte proliferation, migration, and secretion in vitro Pubmed: 32224529
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPA007Po01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA007Po01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
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MAA007Po21 Monoclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
SEA007Po ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA007Po Multiplex Assay Kit for Angiogenin (ANG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA007Po01 ELISA Kit DIY Materials for Angiogenin (ANG) Main materials for "Do It(ELISA Kit) Yourself"