ELISA Kit DIY Materials for Fibronectin (FN)
FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein
- Product No.KSA037Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.69ng/mL.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 860
For more details, please contact local distributors! US$ 1434 US$ 2581 US$ 5019 US$ 8604
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Fibronectin (FN).No significant cross-reactivity or interference between Fibronectin (FN) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
American Journal of Physiology - Renal Physiology | Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase PubMed: 19019916 |
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PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592 |
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Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132 |
Biomedical Materials | In vivo PubMed: 25634132 |
American Journal of Medical and Biological Research | Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease Ajmbr: 3 |
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journal of perinatal medicine | Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. pubmed:27865093 |
Chinese Journal of Contemporary Neurology and Neurosurgery | Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke Article_en:CJFDTotal-XDJB201611014 |
Scientific Reports | Serum fbronectin distinguishes the early stages of hepatocellular carcinoma pubmed:28842594 |
Molecular Medicine Reports | Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression 10.3892/mmr.2017.7563 |
Cell Death & Disease | Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. pubmed:29022912 |
Journal of Cellular Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits Pubmed:29932250 |
Biomedicine & Pharmacotherapy | Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities Pubmed: 30530231 |
Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809 |
Inflammation | TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling Pubmed: 31111299 |
EBioMedicine | Three kinds of corneal host cells contribute differently to corneal neovascularization |
Renal Failure | Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy Pubmed: 31352855 |
Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328 |
Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair Pubmed: 31758411 |
FASEB Journal | Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis Pubmed: 31930571 |
Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort Pubmed: 32210109 |
International Journal of Women¡¯s Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? |
Advanced glycation end-products associate with podocytopathy in type II diabetic patients | |
IBRO Neuroscience Reports | Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function Pubmed:35586775 |
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KSA037Hu01 | ELISA Kit DIY Materials for Fibronectin (FN) | Main materials for "Do It (ELISA Kit) Yourself". |