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ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP)
aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
- Product No.KSA153Po01
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.115ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1361
US$ 2268
US$ 4082
US$ 7938
US$ 13608
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761 |
| Food and Nutrition Sciences | Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684 |
| Diabetes Research and Clinical Practice | Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520 |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700 |
| Annals of Hepatology | Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source |
| Biomedicine & Aging Pathology | Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348 |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413 |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529 |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source |
| PLOS ONE | Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126 |
| BMC Med Imaging | Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587 |
| Tumour Biol | Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893 |
| Acta Veterinaria Brno | The concentrations of selected blood serum proteins in calves during the first three months of life 85:33 |
| Hepatobiliary Pancreat Dis Int | Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf |
| Frontiers in Physiology | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats v04n07a07a |
| International Journal of Clinical and Experimental Medicine | Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model 1345-1349 |
| Tumor Biology | Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway pubmed:28651490 |
| Advances in Clinical and Experimental Medicine | Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles ISSN 2451-2680 |
| Tumor biology | Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats pubmed:28720064 |
| EBioMedicine | Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma Pubmed:30045829 |
| Sensors and Actuators B: Chemical | One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein 10.1016:j.snb.2018.03.038 |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302 |
| Journal of Investigative Surgery | Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation Pubmed:28107094 |
| Asian Pacific Journal of Cancer Prevention | Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats Pubmed:29693337 |
| Antioxid Redox Signal | The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma Pubmed:29687726 |
| Cancer Biology | Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer |
| Cancer Biology | Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine |
| Biomedicine & Pharmacotherapy | The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932 |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma |
| American Journal of Transplantation | Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence Pubmed: 31162867 |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311 |
| Hepatology Research | Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385 |
| REDOX REPORT | Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … Pubmed: 32172678 |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440 |
| Nutr Cancer | Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation Pubmed: 32718197 |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo | |
| DEVELOPMENT | Blocking estrogen-induced AMH expression is crucial for normal follicle formation 33658225 |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023 |
| Journal of Gastrointestinal Cancer | In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC 34698995 |
| Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA153Po01 | Recombinant Alpha-Fetoprotein (AFP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA153Po02 | Recombinant Alpha-Fetoprotein (AFP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA153Po03 | Recombinant Alpha-Fetoprotein (AFP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA153Po02 | Polyclonal Antibody to Alpha-Fetoprotein (AFP) | WB; IHC; ICC; IP. |
| PAA153Po01 | Polyclonal Antibody to Alpha-Fetoprotein (AFP) | WB; IHC; ICC; IP. |
| PAA153Po03 | Polyclonal Antibody to Alpha-Fetoprotein (AFP) | WB; IHC; ICC; IP. |
| LAA153Po81 | FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP) | WB; IHC; ICC; IF. |
| LAA153Po71 | Biotin-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP) | WB; IHC; ICC. |
| SEA153Po | ELISA Kit for Alpha-Fetoprotein (AFP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA153Po | Mini Samples ELISA Kit for Alpha-Fetoprotein (AFP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA153Po | Multiplex Assay Kit for Alpha-Fetoprotein (AFP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA153Po01 | ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP) | Main materials for "Do It (ELISA Kit) Yourself". |