ELISA Kit DIY Materials for Ubiquitin (Ub)

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

  • Product No.KSA164Mi01
  • Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400ng/mL
  • Applicable Sensitivity2.63ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1224  US$ 2040  US$ 3672  US$ 7140  US$ 12240 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ubiquitin (Ub). No significant cross-reactivity or interference between Ubiquitin (Ub) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Breast Cancer Res Treat Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970
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Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
RPA164Mi01 Recombinant Ubiquitin (Ub) Positive Control; Immunogen; SDS-PAGE; WB.
RPA164Mi02 Recombinant Ubiquitin (Ub) Positive Control; Immunogen; SDS-PAGE; WB.
CPA164Mi21 OVA Conjugated Ubiquitin (Ub) Immunogen; SDS-PAGE; WB.
PAA164Mi01 Polyclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
PAA164Mi08 Polyclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
PAA164Mi02 Polyclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
MAA164Mi22 Monoclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
MAA164Mi21 Monoclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
MAA164Mi26 Monoclonal Antibody to Ubiquitin (Ub) WB; IHC; ICC; IP.
SEA164Mi ELISA Kit for Ubiquitin (Ub) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA164Mi ELISA Kit for Ubiquitin (Ub) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA164Mi Multiplex Assay Kit for Ubiquitin (Ub) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA164Mi01 ELISA Kit DIY Materials for Ubiquitin (Ub) Main materials for "Do It (ELISA Kit) Yourself".
KSA164Mi11 ELISA Kit DIY Materials for Ubiquitin (Ub) Main materials for "Do It (ELISA Kit) Yourself".
CGA164Mi02 cDNA of Ubiquitin (Ub) PCR; Protein Expression; etc.