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ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH)

α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

  • Product No.KSA239Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.5-10,000pg/mL
  • Applicable Sensitivity48.1pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Melanocyte Stimulating Hormone (aMSH). No significant cross-reactivity or interference between Alpha-Melanocyte Stimulating Hormone (aMSH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Biochem. Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone. Pubmed: 23341471
Pigment Cell Melanoma Res Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light PubMed: 26582755
The Journal of Biological Chemistry Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice Pubmed:27080257
Pigment Cell & Melanoma Research Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light Pubmed:26582755
Nature Communications Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives. pubmed:28794470
Cell Stem Cell Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses Pubmed:29681516
European Neuropsychopharmacology Epigenetic alterations of the POMC promoter in tobacco dependence Pubmed:29871818
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637
Neuropsychobiology Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence Pubmed: 31117084
INTERNATIONAL IMMUNOPHARMACOLOGY Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathway Pubmed: 32892076
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CEA239Ra ELISA Kit for Alpha-Melanocyte Stimulating Hormone (aMSH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA239Ra Multiplex Assay Kit for Alpha-Melanocyte Stimulating Hormone (aMSH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA239Ra11 ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH) Main materials for "Do It (ELISA Kit) Yourself".