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ELISA Kit DIY Materials for Nesfatin 1 (NES1)

  • Product No.KSA242Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range617.3-50000pg/mL
  • Applicable Sensitivity251.4pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1). No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Bulletin of Clinical Psychopharmacology Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder Psikofarmakoloji: 2043
Progress in Neuro-Psychopharmacology & Biological Psychiatry High plasma nesfatin-1 level in patients with major depressive disorder PubMed: 21163317
Endocrine Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus SpringerLink: h978088657555w16
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
Psychoneuroendocrinology. Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder PubMed: 22595767
Iranian Journal of Health and Physical activity Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats Ijvst: Source
European Journal of Obstetrics & Gynecology and Reproductive Biology Decreased serum nesfatin-1 levels in endometriosis Pubmed: 24702902
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. Psikofarmakoloji: Source
Current Pharmaceutical Design Role of NUCB2/nesfatin-1 as a possible biomarker. Pubmed: 23537082
Regulatory, Integrative and Comparative Physiology A role of nesfatin-1/NucB2 in dehydration-induced anorexia Pubmed:24829503
J Endocrinol Invest. Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome Pubmed:24920281
Int J Surg. The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. Pubmed:25448651
Hormone molecular biology and clinical investigation The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers Pubmed:25581765
Turk J Gastroenterol. New hormones to predict the severity of gallstone-induced acute pancreatitis Pubmed:25599787
Respiration Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? PubMed: 26088934
J Clin Res Pediatr Endocninol Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children PubMed: 26831553
Bosn J Basic Med Sci Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome PubMed: 26295295
Original Article / Orijinal Makale Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 Sayilar: 294
Gynecol Endocrinol Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome PubMed: 26062107
International Journal of Neuroscience Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* Pubmed:26872406
未找到 Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment P.1.e.012.pdf
International Journal of Developmental Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage. pubmed:26872406
Current Pharmaceutical Design Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease pubmed:28137218
Arch Endocrinol Metab Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes pubmed:28977160
Indian Journal of Psychiatry Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? pubmed:28529367
Biomedicine & Pharmacotherapy Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ Pubmed:29604590
Biochimica et Biophysica Acta Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling Pubmed:29627363
BMC Psychiatry Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients Pubmed:29615007
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744
An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
clinical neurology and neurosurgery Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels Pubmed: 32311618
Ann Nutr Metab Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789
Endokrynologia Polska Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke 33283259
Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder
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KSA242Hu11 ELISA Kit DIY Materials for Nesfatin 1 (NES1) Main materials for "Do It (ELISA Kit) Yourself".