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ELISA Kit DIY Materials for Histone H3 (H3)
- Product No.KSA285Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.115ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1128
US$ 1880
US$ 3384
US$ 6580
US$ 11280
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Histone H3 (H3). No significant cross-reactivity or interference between Histone H3 (H3) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890 |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA285Hu01 | Active Histone H3 (H3) | Cell culture; Activity Assays. |
| RPA285Hu01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA285Hu01 | Anti-Histone H3 (H3) Polyclonal Antibody | Loading Control of WB. |
| PAA285Hu01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| CAA285Hu22 | Anti-Histone H3 (H3) Monoclonal Antibody | Loading Control of WB. |
| KSA285Hu01 | ELISA Kit DIY Materials for Histone H3 (H3) | Main materials for "Do It (ELISA Kit) Yourself". |