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ELISA Kit DIY Materials for Mucin 1 (MUC1)

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

  • Product No.KSA413Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.135ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 1 (MUC1). No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734
J Hum Lact Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization PubMed: 26116637
Breast Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295
PLoS One. The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids Pubmed:27182834
Int J Clin Exp Med The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf
Talanta Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification. pubmed:28107977
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
Bio-protocol Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA 10.21769:BioProtoc.2879
Biosensors and Bioelectronics Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review
Journal of Electroanalytical Chemistry A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA413Ra01 Recombinant Mucin 1 (MUC1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA413Ra02 Recombinant Mucin 1 (MUC1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA413Ra01 Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
PAA413Ra02 Polyclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
MAA413Ra21 Monoclonal Antibody to Mucin 1 (MUC1) WB; IHC; ICC; IP.
SEA413Ra ELISA Kit for Mucin 1 (MUC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA413Ra Multiplex Assay Kit for Mucin 1 (MUC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA413Ra01 ELISA Kit DIY Materials for Mucin 1 (MUC1) Main materials for "Do It(ELISA Kit) Yourself"